Antitumour and antimicrobial activities of chloro derivatives of Synthetic Curcumin and their metal chelates.

Abstract

Curcumin and its analogues have been extensively studied as effective medicine for the treatment of cancer and related diseases. Recently several curcuminoid analogues were synthesized and their chemotherapeutic potential has been revealed. In the present study, the synthesis and characterization of three new chlorine containing curcuminoid analogues and their metal chelates Cu(II) and Al(III) are discussed here. The curcuminoid analogues namely 1,7-di(4-chloro phenyl)-1,6-heptadiene-3,5-dione(HL1), 1,7- di(2-chloro phenyl)-1,6-heptadiene-3,5-dione(HL2), and 1,7-bis(3,4 dichloro phenyl)-1,6-heptadiene-3,5-dione(HL3) and their Cu(II) and Al(III) chelates were synthesized and were characterized using UV, IR, 1H NMR and mass spectral data. In vitro cytotoxic studis were done with ligand and metal complexes (Cu and Al) against DLA and EAC cells using Tryptan blue exclusion method and antibacterial study of the compounds were done using agar well diffusion method. The in vivo antitumour activity of the ligand and complexes were determined by using DLA cells in mice and compared with standard anticancer drug cyclophosphamide. The life span of the treated animals were increased upto 60-70%. The present investigation reveals that the Cu(II) complexes show enhanced cytotoxic activity where as the Al(III) complexes have greater activity towards in vivo antitumour studies and antibacterial studies.