Potential of Ribosome-inactivating proteins (RIPs) of Mirabilis jalapaL. as an antiacne: Effect on Proliferation of CulturedSebocyte Cells and its Antibacterial Activities againstPropionibacteriumacnesand Staphylococcus epidermidis

Abstract

Previous researches showed that Ribosome-inactivating proteins (RIPs) isolated from Mirabilis jalapa L. demonstrated some bioactivities such as in vitro cytotoxic on cancer cell lines, antiinflamation and inhibition of nodule growth in animal model. Therefore, this protein hasa potentialto be developed as antiacne. Pathogenesis of acne is caused by some factors such as growth of sebocyte cells that produce sebum and infection caused by bacteria of Propionibacteriumacnes and Staphylococcus epidermidis .This research was aimed to determine whether the extract has an effect on proliferation of primary cultured sebocyte cells and whether the extract possess antibacterial activities against Propionibacteriumacnes and Staphylococcus epidermidis .Result of this study could have a benefit in the further development of use of protein of M. jalapa as anti acne. RIPs of M. jalapa leaves were extracted using phosphate buffer. This protein fraction was then determined in its activity on cleaving of supercoiled double stranded plasmid DNA in order to identify presence of RIPs in the extracts. The protein fraction was then testedon cultured sebocyte cells and on itsantibacterial activity against Propionibacteriumacnes and Staphylococcus epidermidis . Results demonstrated that the protein extract at concentration of 0.3-2.5 mg/mL has inhibition activity on proliferation of cultured sebocyte cells. The protein extract hasbacteriostaticeffect on growth of Staphylococcus epidermidis (MIC 10 mg/mL).