First order derivative and dual wavelength spectrophotometry methods development and validation for simultaneous estimation of alogliptin and pioglitazone in bulk and dosage form

Abstract

Simple, sensitive, rapid and accurate UV spectroscopic methods have been developed for the Simultaneous estimation of Alogliptin and Pioglitazone bulk and pharmaceutical dosage forms. First order derivative and Dual wavelength methods were developed and validated using solvent methanol. Both methods show linearity at 5-30 g/ml. The first order derivative spectra of each solution were obtained. ZCP of Alogliptin was found 275.60 nm and ZCP of Pioglitazone was found 268.20 nm. The zero crossing point (ZCP) of Alogliptin at which Pioglitazone is measured and ZCP of Pioglitazone at which Alogliptin is measured. In Dual wavelength method, spectra two wavelengths 270.20 nm and 265 were selected as ? ₁ and ? ₂ for the estimation of Alogliptin. Pioglitazone shows the same absorbance at these wavelengths. Similarly, wavelengths 280 nm and 271 nm were selected as ? ₃ and ? ₄ for estimation of Pioglitazone. Alogliptin shows the same absorbance at these wavelengths. The methods were validated based on ICH guidelines. There are simple, sensitive, and reliable and results are reproducible for the routine analysis of Alogliptin and Pioglitazone.