RP-HPLC method for estimation of Gemcitabine HCl 1gm injection in parenteral dosage form

Authors

  • M. R. Jayapal Institute of Aeronautical engineering college Hyd, Affiliated to (JNTU-Hyderabad)
  • A Santosh Kumar Sreevatsav Department of Pharmaceutical Analysis, MRR College of Pharmacy, Nadergul, A.P.
  • K. Shyam Sunder Department of Pharmaceutical chemistry, MLR Institute of Pharmacy, Dundigal (V), Hyd-43

DOI:

https://doi.org/10.7439/ijpc.v3i3.44

Abstract

A Simple, precise, rapid and stability indicating reverse phase liquid chromatography method has been developed and validated in order to determine Gemcitabine HCl in parenteral dosage form. A L-7 column made up ot stainless steel having C-18 as stationary phase with an internal diameter of 250 X 4.6mm is used. Phosphate buffer : methanol in the ration of 97:3 is used as the mobile phase using 0.2l membrane filter. A UV detector is used where 266nm is the wavelength set for detection with a flow rate of 1.0ml/min and the runtime was for 10 min. The method is then validated for linearity, selectivity, precision (method, intermediate, system), robustness. These parameters generated results which are under the limits.

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Published

2013-10-01

Issue

Vol. 3 No. 3 (2013): Jul- Sep

Section

Research Articles

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How to Cite

1.
RP-HPLC method for estimation of Gemcitabine HCl 1gm injection in parenteral dosage form. Int J of Pharm Chem [Internet]. 2013 Oct. 1 [cited 2025 Jun. 10];3(3):57-66. Available from: https://ssjournals.co.in/index.php/ijpc/article/view/1309
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