RP-HPLC method for estimation of Gemcitabine HCl 1gm injection in parenteral dosage form

Authors

  • M. R. Jayapal Institute of Aeronautical engineering college Hyd, Affiliated to (JNTU-Hyderabad)
  • A Santosh Kumar Sreevatsav Department of Pharmaceutical Analysis, MRR College of Pharmacy, Nadergul, A.P.
  • K. Shyam Sunder Department of Pharmaceutical chemistry, MLR Institute of Pharmacy, Dundigal (V), Hyd-43

DOI:

https://doi.org/10.7439/ijpc.v3i3.44

Abstract

A Simple, precise, rapid and stability indicating reverse phase liquid chromatography method has been developed and validated in order to determine Gemcitabine HCl in parenteral dosage form. A L-7 column made up ot stainless steel having C-18 as stationary phase with an internal diameter of 250 X 4.6mm is used. Phosphate buffer : methanol in the ration of 97:3 is used as the mobile phase using 0.2l membrane filter. A UV detector is used where 266nm is the wavelength set for detection with a flow rate of 1.0ml/min and the runtime was for 10 min. The method is then validated for linearity, selectivity, precision (method, intermediate, system), robustness. These parameters generated results which are under the limits.

Downloads

Downloads

Published

2013-10-01

Issue

Vol. 3 No. 3 (2013): Jul- Sep

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
  • The author must submit Copyright form After acceptance of the article.

How to Cite

1.
RP-HPLC method for estimation of Gemcitabine HCl 1gm injection in parenteral dosage form. Int J of Pharm Chem [Internet]. 2013 Oct. 1 [cited 2025 Jun. 20];3(3):57-66. Available from: https://ssjournals.co.in/index.php/ijpc/article/view/1309
Crossref
Scopus
Google Scholar
Europe PMC