Bioactivity of 6-Aryl-4,5-dihydropyridazin-3(2H)-ones Using Brine Shrimp (Artemia salina) Lethality Assay

Abstract

Pyridazine is an important component of various biological active synthetic compounds which can leads to the development of novel and safe medicinal agents. In the present study, some 6-aryl-4,5-dihydropyridazin-3(2H)-one derivatives ( 2a2f ) were prepared from aroyl propionic acids ( 1a-1f ). The final compounds ( 2a2f ) were evaluated for cyotoxicity activity by brine shrimp assay method. All the 6 compounds ( 2a2f ) were tested at dose level 5, 10, 20 (g/ML) and potassium dichromate at dose level 10, 20, 30 (g/ML) as reference drug. Compound 2c and 2e exhibited potent brine shrimp lethality with LC ₅₀ 1.023 and 1.20 ?g respectively. Other compounds 2a , 2b , 2d , and 2f have also showed significant cytotoxicity with LC50 7.58, 6.76, 8.91 and 4.46 ?g respectively. The present study supports that brine shrimp bioassay is simple reliable and convenient method for assessment of bioactivity of 2a-2f and lends support for their uses in medicine.