Development and validation of a reversed-phase HPLC method for the determination of Anagrelide in capsule

Authors

  • R. Kalaichelvi K.C.Reddy Institute of Pharmaceutical Sciences, Jangamguntla Palem, Medikonduru Mandal, Guntur-522 348
  • E. Jayachandran S.C.S. College of Pharmacy, Harapanahalli-583131. Devanagari Dist, Karnataka

DOI:

https://doi.org/10.7439/ijpc.v3i1.30

Keywords:

Women beliefs, nutrition, diet during pregnancy and lactation (post partum)

Abstract

Development and validation of a simple and sensitive reversed-phase high performance liquid chromatorgaphic (RP-HPLC) procedure for the analysis of Anagrelide in tablet dosage forms is described. The drug was separated on Agilent Eclipse XDB C18, 150 x 4.6 mm, 5m column and UV detection carried out at 250 nm. The elution was achieved isocratically with a mobile phase mixture of acid buffer (pH 4) and acetonitrile in the ratio of 70:30%v/v. The retention time for anagrelide was 5.8 min. The recovery of the drug in HPLC was 99.63 to 100.50 %. The method was validated by doing specificity, precision, linearity, accuracy, LOD, LOQ and range.

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Author Biography

  • R. Kalaichelvi, K.C.Reddy Institute of Pharmaceutical Sciences, Jangamguntla Palem, Medikonduru Mandal, Guntur-522 348
    Assistant Professor

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Published

2013-03-01

Issue

Vol. 3 No. 1 (2013): Jan-Mar

Section

Research Articles

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How to Cite

1.
Development and validation of a reversed-phase HPLC method for the determination of Anagrelide in capsule. Int J of Pharm Chem [Internet]. 2013 Mar. 1 [cited 2026 Jan. 7];3(1):01-3. Available from: https://ssjournals.co.in/index.php/ijpc/article/view/1300
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