METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OXYBENZONE, OCTINOXATE AND AVOBENZONE IN SUNSCREEN LOTION BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • Tapan Banker ICPA Health Products Ltd, 286/287 G.I.D.C, Ankleshwar, Dist: Bharuch, Gujarat
  • Prashant Kale ICPA Health Products Ltd, 286/287 G.I.D.C, Ankleshwar, Dist: Bharuch, Gujarat
  • Ashok Peepliwal ICPA Health Products Ltd, 286/287 G.I.D.C, Ankleshwar, Dist: Bharuch, Gujarat

DOI:

https://doi.org/10.7439/ijbar.v2i2.25

Abstract

A simple, precise, accurate, selective, and economic reversed-phase RP-HPLC method has been developed for simultaneous analysis of Oxybenzone, Octinoxate and Avobenzone in sun-screen lotion. Chromatographic separation was performed by using UV detector at wavelength of 330nm. The separation was performed at ambient temperature by using C18 stationary phase followed by methanol: phosphate buffer (90:10) pH (3.0) as a mobile phase.
The mobile phase flow rate was 1.2ml/min and gradient elution was performed. Total run time was 25min. The method was successfully applied on formulation and there has been no
interference of matrix components. The linearity of method was obtained in the range of 18 to 48

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Published

2010-03-01

Issue

Vol. 2 No. 2 (2011): Feb

Section

Original Research Articles

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How to Cite

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OXYBENZONE, OCTINOXATE AND AVOBENZONE IN SUNSCREEN LOTION BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. (2010). International Journal of Biomedical and Advance Research, 2(2), 92-102. https://doi.org/10.7439/ijbar.v2i2.25
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