Association of Soluble ? - Klotho and Klotho Polymorphisms (KL-VS and G-395A) with Essential Hypertension in Indian Population
DOI:
https://doi.org/10.7439/ijbar.v9i12.4969Keywords:
Gene polymorphism, ? - Klotho, anti - aging protein, Cardiovascular diseaseAbstract
Klotho, a novel anti-aging gene, is known to ameliorate aging phenotypes and extend lifespan. Recent studies have highlighted arterial aging as a risk factor for hypertension. Thus, Klotho appears to be a link between aging and cardiovascular diseases (CVD) including hypertension. KL-VS and G-395A polymorphisms of Klotho and its soluble levels have been associated with CVD incidence. We analyzed the prevalence of alfa-Klotho polymorphisms KL-VS and G-395A and their influence on soluble alfa-Klotho levels in essential hypertension (EH) patients in Indian population. One-hundred-twelve EH patients and age, BMI-matched 103 normotensive controls were recruited. Soluble alfa-Klotho levels were measured by Enzyme-Linked-Immunosorbent-Assay. Genotyping of single nucleotide polymorphisms (SNP) was performed using TaqMan assays. Soluble alfa-Klotho levels in patients were significantly lower (26.9%,p=0.001) than controls. ROC curve analysis for soluble α-Klotho showed a cut-off of 580pg/ml with AUC 0.798(SE=0.031, 95%CI=0.737-0.859). Genotype distributions for both polymorphisms did not differ between the groups. Distribution of soluble alfa-Klotho levels in both groups did not vary across KL-VS genotypes. In patient group, GA and AA genotypes of G-395A polymorphism were associated with significantly lower (15.4%,p=0.014) soluble alfa-Klotho levels compared to GG genotype. Soluble alfa-Klotho levels were significantly lower in EH patients as compared to healthy normotensive individuals.
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