Effect of vitamin E on hydrogen peroxide and nitrogen levels in male wistar albino rats infected with trypanosoma Brucei Brucei
DOI:
https://doi.org/10.7439/ijbar.v10i01.4934Keywords:
Vitamin E, Trypanosomiasis, Nitric Oxide, Hydrogen PeroxideAbstract
This research work was conducted to assess the antioxidant effect of vitamin E on hydrogen peroxide and nitrogen oxide levels. 24 albino rats were divided randomly into six groups of four test animal per group. Group A served as control and were given normal rat chow and water. Group B served as trypanosome treated and were infected with 1×106 trypanosome. Group C were infected with 1×106 trypanosome and treated with the standard drug (diaminazene acetuate). Group D were infected with 1×106 trypanosome and treated with 0.1mg/kg body weight of vitamin E (low dose). Group E were infected with 1× 106 trypanosome and treated with 0.5mg/kg body weight of vitamin E (moderate dose). Group F were infected with 1×106 trypanosome and treated with 1.0mg/kg body weight (high dose) for 14 days. Hydrogen peroxide and nitric oxide were assayed by scavenging Assay methods. The result of this study shows that hydrogen peroxide levels showed a significant difference (p 0.05) when compared with value of 0.31
Downloads
References
Kalu AU., Oboegbulem SI., Uzoukwu M. Trypanosomosis in small ruminants maintained by low riverine tsetse population in central Nigeria. Small Ruminant Research 2001; 40(2):109-115.
Njiokou F., Simo G., Nkinin S.W., Laveissiere C. & Herder S. Infection rate of Trypanosoma brucei s.1, T. vivax, T. congolense “forest type and T. simiae in small wild vertebrate in south Cameroon. Acta Tropica 2004; 92:139-146
Anosa VO, Kaneko JJ. Pathogenesis of T brucei infection in deer mice (P. maniculatus).Ultra structural pathology of the spleen, liver, heart and kidney. Veterinare Pathology, 1984; 21: 229-237.
Bruijn JA, Oemar BS, Ehrick HH, Foidart JM, Flueures GJ. Antibasement membrane glomerulopathy in experimental trypanosomiasis. Journal of Immunology.1987; 139: 2482- 2485.
Damian RT. Parasite immune evasion and exploitation: reflections and projections. Parasitology, 1997; 115: SI69-S175.
Anosa VO. Haematological and biochemical changes in human and animal trypanosomiasis. Rev. Elev. Med. Vet. Pays Trop 1988; 41(42): 151-164.
Ekanem, J.T. and Yusuf, O.K. Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats. Afr. J. Biomed. Res., 2008; 11: 79-85.
Akanji, M.A., Adeyemi, O.S., Oguntoye, S.O. and Sulaiman, F.A. Psidium Guajava extract reduces trypanosomosis associated lipid peroxidation and raises glutathione concentrations in infected animals. EXCLI Journal. 2009; 8:148-154.
Brigelius-Flohe. Tissue-specific functions of individual glutathione peroxidases. Free Radic Biol Med. 1999; 27(9-10): 951-65.
Packer L, Kraemer K, Rimbach G. Molecular aspects of lipoic acid in the prevention of diabetes complications. Nutrition 2001; 17: 888-95.
Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in Human Health: Current status and future prospects. J Assoc Physicians India. 2004; 52: 794-803.
Meshnick SR, Chance KP, Cerami A. Haemolysis of the blood stream forms of T. brucei. Biochem. Pharmcol. 1977; 26: 1923.
Igbokwe IO, Esievo KAN, Saror DI, Obagaiye OK. Increased susceptibility of erythrocytes to in vitro peroxidation in acute T. brucei infection in mice. Vet. Parasitol. 1994; 55: 279-286.
Ihedioha JI, Anwa AP. Liver retinol and carotenoid concentration of rats experimentally infected with Trypanosoma brucei. Trop. Vet. 2002; 20(1): 1-7.
Fairlamb AH. Biochemistry of trypanosomes. Rational approach to chemotherapy. TIBS. 1982; 7 (7): 249-253.
Karber G. Reihenversucbe. Arch Exper Pathol Pharmacol 1991; 162: 480-483.
Herbert, W.J.; Lumsden, W.H. Trypanosoma brucei: A Rapid Matching method for estimating the host’s parasitaemia. Exp. Parasitol., 1976; 40: 427-431.
Ruch RJ, Cheng SJ, Klaunig JE. Prevention of Cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989; 10: 1003-1008.
Marcocci L., Maguire J. J., Droylefaix M. T., Packer L. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem. Biophys. Res. Commun. 1994; 201: 748-755.
Lai L. S., Chou S. T., Chao W. W. Studies on the antioxidative activities of Hsian-tsao (Mesonaprocumbens Hemsl) leaf gum. J. Agric. Food Chem. 2001; 49: 963-968.
Shibuki K., Okada D. Endogenous nitric oxide release required for long-term synaptic depression in the cerebellum. Nature 1991; 349: 326-328.
Bredt D. S., Snyder S. H. Nitric oxide: a physiologic messenger molecule. Annu. Rev. Biochem. 1994; 63: 175-195.
Liu R. H. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr. 2003; 78: 517S-520S.
Umar IA, Wuro-Chekke AU, Gidado A, Igbokwe IO. Effects of combined parenteral vitamin C and E administration on the severity of anaemia, hepatic and renal damage in T. brucei infected rabbits. Vet. Parasitol. 1999; 85: 43-4.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 International Journal of Biomedical and Advance Research
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
How to Cite
