A Effect of Vitamin E on glutathione reductase and reduced glutathione levels of male wistar albino rats infected with Trypanosomabruceibrucei

Authors

  • Adebayo Olugbenga Adegoke Department of Medical Laboratory Science, madonna University
  • Johnpaul Ikechukwu Chukwu Department of Medical Laboratory Science, College of Medicine, University of Nigeria
  • Nnenna Faith Anichukwu Department of Medical Laboratory Science, Madonna University,Elele,Nigeria

DOI:

https://doi.org/10.7439/ijbar.v9i11.4933

Keywords:

Vitamin E, Gluthathione reductase, Trypanosoma

Abstract

The research work was carried out to assess the antioxidant effect of Vitamin E on glutathione reductase and reduced glutathione levels of male wistar albino rats infected with Trypanosomabruceibrucei . 24 albino rats were divided into six groups of four test animal per group namely: control, Trypanosome infected, Trypanosome + diaminazine treated Trypanosome + 0.1mg Vitamin E, Trypanosome + 0.5mg Vitamin E and Trypanosome + 1.0mg Vitamin E respectively. Glutathione reductase and reduced glutathione were determined in all albino rats using oxidation -Reduction process .The data was subjected to statistical analysis using SPSS version 20. The result of this study shows that there was no significant difference (p>0.05) in reduced glutathione (

Downloads

References

Adamu, U. O. Haruna, M. K., Orbagbedia R. P., Benjamin W., Nwezor F.N.C., and MuhammedM. (2011). Control of African Trypanosomiasis in Nigeria. Time to strengthening Integrated Approches(A Review); International Journal of Animal and VeterinaryAdvances3 (3): 138- 143.

Anene, B.M., Ezeokonkwo, R.C., Mmesirionye, T.I., Tetley, J.N.A., Brook, J.M.,andDekoning, H.P. (2006). A diminazene-resistant strain of Trypanosomabruceibruceiisolated from a dog is cross-resistant to Pentamidine in experimentally infected albino rats. Parasitology,132,127-133.

Anika S. M., Shetty S.N., Asuzu, I. U, and Chime A. B. (1987). Effect of some trypanocides and anti-inflammatory agents in experimental Trypanosomabrucei Infection in mice.Tropical Biomedical journal,3:523-531.

Boid, R., El.Amin, E.A., Mahmoud, M.M., and Lukins, A.G. (2011).Trypanosomaevansi infections and anti-bodies in goats, sheep and camels in Sudan.Tropical Animal Health Production,13, 141.

Dietrich, M., Traber, M.G., Jacques, P.F., Cross, C.E., Hu, Y., and Block G. (2006). Does γ-tocopherol play a role in the primary prevention of heart disease and cancer? A review.American Journal Coll Nutrition, 25,292-299.

Ellman GL (1959). Tissue sulphydryl groups. Arch Biochem Biophys ; 82: 70-77.

Herbert, W.J. and W.H.R. Lumsden, 1976. Trypanosomabrucei: A rapid matching method for estimating the host's parasitemia. Exp. Parasitol., 40: 427-431.

Kahn C. M.(2005) Blood parasites.The Merck Veterinary Manual, Merck & Co. inc.Williamson J. (1970) review of chemotherapeutic and chemoprophylactic agents. In Mulligan H. W., Potts, W. H.(Eds).The African Trypanosomiases, Allien and Unwin Ltd, London, 12:116-141

Kakkar P, Das B, Viswanathan, P.N (1984), Indian J BiochemBiophys, 2: 130-132

Karber, G. (1931), "BeitragzurkollektivenBehandlungpharmakologischerReihenversuche; Archiv fur ExperimentellePathologie und Pharmakologie, 162,480-7.

Osmar, A. S, Jenning F. W, Holmes P. H., (1992) Rapid Development of drug resistant Trypanosomaevansi in immunosuppressed mice. Acta Tropica:50:249-255.

Sapakal VD, Shikalgar TS, Ghadge RV, Adnaik RS, Naikwade NS, Magdum CS (2008). In vivo screening of antioxidant profile: A review. J Herb Med Toxicol. 2:1-8.

Sen, C.K., Khanna, S. and Roy, S. (2006). Tocotrienols: vitamin E beyond tocopherols. Life Science,78,2088-2098.

Sulaiman, F. A., and Adeyemi, O. S., (2010) changes in Haematological indices and protein concentration in Trypanosomabrucei infected rats treated with Homidium Chloride and Diminazeneaceturate. E. X. C. L. I Journal9:39-45.

Traber.M.G. (2007).Vitamin E regulatory mechanisms. Annual Revisied Nutrition,27:347-362.

Umar I.A. et al.,(2007).Amelioration of anaemia and organ damage by combined intraperitoneal administration of vitamin A and C to Trypanosomalbruceibrucei infected rats .African Journal of Biotechnology, 6,2083-2066.

Umar I A, Rumah BL, Asueliman BI, Mazai MH, Ibrahim MA, Isah S (2008): Effects of intraperitoneal administration of vitamins B12 and E combinations on the severity of Trypanosomabruceibruceiinfection in rats. African Journal of Biochemical Research,2 (3): 088-091

Verhagen, H., Buijsse, B., Jansen and Bueno-de-Mesquita, B. (2006).The state of antioxidant affairs.Nutrution of Today,41,244-250.

WHO (World Health Organization) (2006). African trypanosomiasis (sleeping sickness) .

Yakubu,D.P., Dawet, A.; Olaleye N. A. (2014).Effects of vitamin E and selenium on some blood parameters of Trypanosomabruceibrucei infected rats.British journal of applied science and technology, 4(7): 1100-1108.

Yusuf,A.B.,Umar, I.A.,MusaU.B.,andNok A.J.(2012).Screening of Veramygdalina and Hymenocardiaacida extracts and 1-3-diaminopropane for antitrypanosomal activity :invitromodel.Journal of medical plants research, 6(19):3573-3578.

Downloads

Published

2018-11-24

Issue

Section

Original Research Articles

How to Cite

A Effect of Vitamin E on glutathione reductase and reduced glutathione levels of male wistar albino rats infected with Trypanosomabruceibrucei. (2018). International Journal of Biomedical and Advance Research, 9(11), 371-375. https://doi.org/10.7439/ijbar.v9i11.4933