Screening of hemoglobinopathies in blood donors (A study of 800 cases)
DOI:
https://doi.org/10.7439/ijbar.v9i10.4927Keywords:
Hemoglobin, sickle cell trait, Thalassemia, HbAbstract
Introduction: Haemoglobinopathies are common genetic disorders of haemoglobin in which there is an abnormal production or structure of the haemoglobin molecule. These hereditary disorders are major public health problems in many parts of the world including South East Asia like India. Prospective prevention through population screening and genetic counselling is the best possible strategy in the prevention of these disorders. The clinical spectrum of these disorders varies from asymptomatic conditions to serious disorders like thalassemia major that requires regular blood transfusions and widespread medical care.
Aim and Objectives: The aim of study is to find out the prevalence of β-thalassemia trait and other haemoglobinopathies in blood donors using the principle of High Performance Liquid Chromatography (HPLC). HPLC is considered as one of the best methods for screening and confirmation of various haemoglobinopathies with rapid, precise and reproducible results.
Material and Method: The study has been carried out as Sample bound study to find the pattern of β -Thalassemia and other Haemoglobinopathies in 800 blood donors, from April 2016 to November2016.The samples were then processed for sickle solubility test, HPLC and Hb electrophoresis for hemoglobinopathies in central clinical laboratory.
Result and Conclusion: In present study, out of 800 blood donors 25 were found to have β thalassemia or other haemoglobinopathy. Among 25 donors with haemoglobinopathies, 16 (61.64%) were having β-thalassemia which was most common followed by sickle cell trait (26.92%) and Hb D Punjab trait(11.54%).
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