Multi-model confirmatory evaluation of anti-inflammatory, analgesic and antioxidant activities of Putranjiva roxburghii wall

Abstract

Putranjiva roxburghii Wall is traditionally used by tribals for treating various health problems. In this study, Analgesic, anti-inflammatory and anti-oxidant activities of ethanolic extract of leaves and stem of Putranjiva roxburghii were evaluated in rats using different evaluatory models for each activity. Carrageenan-induced paw edema, croton oilinduced ear edema and anus edema models were used for evaluating anti-inflammatory activity. Analgesic activity was evaluated by acetic acid induced writhing model, Eddy hot plate model and tail flick models. The plant extract was also evaluated for its In-vitro anti oxidant activity in an attempt to find its potency in providing protection against free radical induced diseases. The results indicates significant dose dependant anti inflammatory activity of ethanolic extract (250 and 500 mg/kg, p.o.) of Putranjiva roxburghii wall with respect to standard drug Indomethacin. The extract showed dose dependent analgesic activity in acetic acid induced writhing model but dose independent activity in case of hot plate and tail flick models as compared to standard drug Indomethacin. The In-vitro antioxidant activity of the ethanolic extract was also significant and comparable with ascorbic acid in both DPPH and H2O2 models.