Hypolipidemic Activity of of Madhuca longifolia in Triton Induced Hyperlipidemic Rats

Abstract

Objective: In the present study, an aqueous extract from Madhuca longifolia bark was evaluated for its hypocholesterolaemic and hypotriglyceridaemic activities using Triton WR-1339 induced hyperlipemic rats as experimental model. Material and Method: Hyperlipidemia was induced by a single injection of Triton WR 1339 (400 mg/kg i.p.) in sprauge dawley rats. Aqueous extract of Madhuca longifolia bark (ML) (250, 500 and 750 mg/kg/day) was administered to hyperlipidemic rats for one week. Harvested serum was analyzed for lipid profile such as cholesterol, triglyceride, and lipoproteins. Oxidative stress parameters like Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Glutathione reductase (GRh) and activity of lipolytic enzyme such as lecithincholesterol-acyltransferase (LCAT) and post-heparin lipolytic activity (PHLA) were estimated in the liver tissues of hyperlipidemic rats. Results: Result of the study suggested that treatment with ML 750mg/kg/day significantly (p?0.01) lowered the level of serum cholesterol, triglyceride phospholipids and increased in lecithincholesterol-acyltransferase activity and post-heparin lipolytic activity compared to Triton-treated rats. In addition, ML 750mg/kg/day significantly (p?0.01) reduced oxidative stress and normalizes the activities of SOD, CAT, GPx and GRh compared to Triton-treated rats. Conclusion: The current study provides strong evidence that intragastric administration of ML 750mg/kg/day has a beneficial effect in treating dyslipidemia with decrease in oxidative stress.