An in silico approach to design T-arms primers for VDR locus to detect osteoporosis
DOI:
https://doi.org/10.7439/ijbar.v8i1.3895Abstract
Osteoporosis is a skeletal disorder characterized by an increased risk of fractures. Studies have shown that BMD and bone turnover are under strong genetic control. The polymorphisms in the Vitamin D receptor (VDR) have been implicated in many studies as a cause of osteoporosis. We aimed at developing a molecular diagnostic kit to detect osteoporosis. We started with designing multiplex primers and finally designed T-ARMS PCR Kit for the VDR locus. We used the currently available softwares for primer designing like PerlPrimer, Primer3, Primer1, BatchPrimer3, WASP, Primer-BLAST and AutoDimer. Our T-ARMS PCR Kit for the VDR locus included the penultimate primer position mismatch to increase the specificity and a universal sequence and tag to assist successful amplification of all primers within the desired annealing temperature range. Our osteoporosis detection kit is an in-silico approach and has to be validated through in vitro PCR amplicifactions.Downloads
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