Detection of biofilm production in Candida species isolates recovered from bloodstream patients

Abstract

Introduction: Candida Bloodstream Infections (CBSIs) are the fourth most common infections among hospitalized patients. Candida biofilm results from an initial attachment of cells to glycoprotein-coated host cells and tissue or biomaterial surfaces. Material and Method: Candida spp. was identified by conventional method and biofilm formation was detected by tube method. Result: C . tropicalis was the most common species followed by C. haemulonii, C. albicans , C. parapsilosis, C. glabrata, C. pelliculosa , C. guilliermondii and C. krusei. 125 of the isolates were tested for biofilm formation of which 67(53.6%) were found to be capable of forming biofilms. Of these 55(82%) biofilm forming isolates were those recovered from patients with Catheter Related Candidemia (CRC) while 12 (17.9%) biofilm forming isolates were without CRC. Most common biofilm producing species were C.pelliculosa followed by C.tropicalis , C.haemulonii , C. parapsilosis , C.glabrata , C. albicans , C.krusie and C. guilliermondii . Conclusion: Our study found more biofilm production in Central Venous Catheter (CVC) Candidemia and most of the CVC related Candidemia were found in diabetic and neutropenic patients and hence care should be taken in those patient who are at risk of developing biofilm production before applying the external appliances.