Isolation and Antimicrobial sensitivity pattern of Klebsiella pneumoniae from sputum samples in a tertiary care hospital
DOI:
https://doi.org/10.7439/ijbar.v7i2.2945Keywords:
Enhanced drug delivery, ethosomes, candidiasis, TEM, vesicleAbstract
Background: Gram negative pathogens are an important cause of community and hospital acquired infections throughout the world. Klebsiella pneumoniae has become one of the more common causes of these infections and one of the important aspects of Klebsiella associated infections is the emergence of multi-drug resistant strains particularly those involved in nosocomial diseases. This study was done to determine the isolation rate of Klebsiella , their antibiogram and for the presence of resistant strains from sputum samples. Materials and Methods: A total number of 128 sputum samples were included in the present study. A total of 128 organisms were isolated, of them 30 (23%) were Klebsiella pneumoniae . Klebsiella were identified by standard microbiological techniques and antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion method and interpreted as per CLSI guidelines. A total of 30 Klebsiella spp were isolated from 128 sputum samples, 100% were Klebsiella pneumoniae , sensitive to Amikacin (66%) Ciprofloxacin (68%), Gentamicin (62%), Cefepime (60%), Imipenem (56.66%), Aztreonam (52.63%). Isolates showed high resistance to Ticarcillin clavulanic acid (81%), Tobramycin (58%), Co-trimoxazole (50%). Results: The present study reveals the frequency of isolation of Klebsiella from sputum samples and their tendency towards antibiotic resistance. K. pneumonia was found to be most sensitive to Amikacin, Gentamicin, Cefepime, Imipenem, Aztreonam and Ciprofloxacin. Females above 60 years of age are more affected and infections are more commonly associated with chronic obstructive pulmonary diseases and pneumonia. Conclusion: The data of this study may be used to determine trends in antimicrobial susceptibilities to formulate local antibiotic policies and overall to assist clinicians in the rational choice of antibiotic therapy.Downloads
References
Asati Rakesh Kumar; Antimicrobial sensitivity pattern of Klebsiella pneumonia isolated from pus from tertiary care hospital and issues related to the rational selection of antimicrobials. Journal of Chemical and Pharmaceutical Research, 2013; 5(11): 326-331.
Steinmann J, Kaase M, Gatermann S, Popp W, Steinmann E, Damman M, Paul A, Saner F, Buer J, Rath P M; Outbreak due to Klebsiella pneumonia strain harbouring KPC-2 and VIM-1 in a German university hospital. Euro Surveill 2011; 16(33): 19944.
Chaudhary B.L., Srivastava Shailja, Singh Brij Nandan and Shukla Snehanshu; Nosocomial Infection due to Multidrug Resistant (MDR) Escherichia coli and Klebsiella pneumoniae in Intensive Care Unit. International Journal of Current Microbiology and Applied Sciences 2014; 3(8):630-635.
Sikarwar Archana Singh and Batra Harsh Vardhan; Prevalence of Antimicrobial Drug Resistance of Klebsiella pneumoniae in India. International Journal of Bioscience, Biochemistry and Bioinformatics 2011; 3(1): 211-215.
Namratha KG, Padiyath Sreeshma, Subbannayya K, Dinesh PV, Hemachandra Champa; Characterization and Antibiogram of Klebsiella spp. Isolated from Clinical Specimen in a Rural Teaching Hospital. Scholars Journal of Applied Medical Sciences (SJAMS) 2015; 3(2E):878-883.
K.N.Ravichitra, P.Hema Prakash, S. Subbarayudu and U. Sreenivasa Rao; Isolation and antibiotic sensitivity of Klebsiella pneumonia from pus, sputum and urine samples. Int. J. Curr. Microbiol. App. Sci 2014; 3(3): 115-119.
Joshua T Freeman, Jessica Nimmo, Eva Gregory, Audrey Tiong, Mary De Almeida, Gary N McAuliffe and Sally A Roberts; Predictors of hospital surface contamination with Extended-spectrum Beeta-lactamase-producing Escherichia coli and Klebsiella pneumonia: patient and organism factors. Antimicrobial Resistance and Infection Control 2014; 3(5):1-7.
Asati Rakesh Kumar; Antimicrobial Sensitivity Pattern of Klebsiella Pneumoniae isolated from Sputum from Tertiary Care Hospital, Surendranagar, Gujarat and Issues Related to the Rational Selection of Antimicrobials. Scholars Journal of Applied Medical Sciences (SJAMS) 2013; 1(6):928-933.
Buddha Bahadur Basnet, Rajan Kumar Dahal, Nabin karmacharya, Basista Prasad Rijal; Retrospective audit of LRTI from sputum samples with respect to Acinetobacter spp., Pseudomonas spp. and Klebsiella spp. From tertiary care Hospital of Nepal. International Journal of Medical and Health Sciences 2013; 2(3):266-274.
Manjula N. G., Girish C. Math., Kavita Nagshetty, Shripad A. Patil, Subhashchandra M. Gaddad, Channappa T. Shivannavar; Antibiotic Susceptibility Pattern of ES?L Producing Klebsiella pneumoniae Isolated from Urine Samples of Pregnant Women in Karnataka. Journal of Clinical and Diagnostic Research 2014; 8(10):8-11.
Shashidhar Vishwanath, Kiran Chawla, Anusha Gopinathan; Multidrug resistant Gram-negative bacilli in lower respiratory tract infections. Iranian Journal of Microbiology 2013; 4(5):323-327.
Osazuwa F, 1Mordi RM, Osazuwa E, Taiwo SS, Alli OAT, Ogbolu DO, Akanni EO, Anukam; Klebsiella has taken lead among uropathogens in University of Benin Teaching Hospital, Benin City, Nigeria-An observation. New York Science Journal. 2010; 3(11):61-64.
Sahly H., Aucken H., Benedi V.J., Forestier C., Fussing V., Hansen D.S., Ofek I., Podschun R., Sirot D., Tomas J.M., Sandvang D., and Ullmann U. Increased Serum Resistance in Klebsiella pneumonia Strains Producing Extended-Spectrum beeta-Lactamases. Antimicrob Agents Chemother 2004; 9(48):3477-3482.
Shah RK, Singh YI, Sanjana RK, Chaudhary N, Saldanha D et al.; Study of extended spectrum beta-lactamases (ESBLs) producing Klebsiella species in various clinical specimens: A preliminary report. Journal of College of Medical Sciences Nepal 2010; 6(3): 19-23.
Shukla I, Tiwari R, Agrawal M; Prevalence of extended spectrum ?-lactamse producing Klebsiella pneumoniae in tertiary care hospital. Indian Journal Medical Microbiology 2004; 22(2): 87-91.
Ali Abdel Rahim KA, Ali Mohamed AM; Prevalence of extended spectrum ?-lactamaseproducing Klebsiella pneumoniae in clinical isolates. Jundishapur Journal of Microbiology 2014; 7(11): e17114.
Kitara LD, Anywar AD, Acullu D, Odongo-Aginya E, Aloyo J, and Fendu M, Afr Health Sci. 2011; (l1): S34
Okonko I.O., Fajobi E.A., Ogunnusi T.A., Ogunjobi A.A. and Obiogbolu C.H. African Journal of Biomedical Research, 2008; 11: 235-250.
Nkang A. O., Okonko I. O, Mejeha O. K., Adewale O. G., Udeze A. O., Fowotade A., E. A. Fajobi, Adedeji A. O. and Babalola E. T. Journal of Microbiology and Antimicrobials 2009; 1(2): 019-026.
Elizabeth B. Hirsch and Vincent H. Tam; Detection and treatment options for Klebsiella pneumonia carbapenemases (KPCs): an emerging cause of multidrug-resistant infection. Journal of Antimicrobial Chemotherapy 2010; 108:1119-1125.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
How to Cite
