Synergistic interactions between Labiatae species and antibiotics on gram positive and gram negative bacterial strains
DOI:
https://doi.org/10.7439/ijbar.v6i10.2603Keywords:
Autoimmune diseases, Biomarker, Drug development, Inflammatory diseases, Personalized medicineAbstract
Objective and methods: This study was aimed to evaluate antibacterial activity; type of interaction between chloroform leaves extract of Mentha piperita, Mentha longifolia and Ocimum basilicum together and with antibiotics by agar well diffusion method on isolated bacterial strain and to determine active constituents responsible on antibacterial activity by agar overlay bioautographic method. Results: Mentha piperita exhibited more pronounced inhibition zone (20mm) against Staphylococcus aureus, Staphylococcus auricularis , Streptococcus mitis and Escherichia coli followed by Mentha longifolia highest inhibition zone (15mm) against Staphylococcus auricularis and Streptococcus mitis , then Ocimum basilicum exhibited highest inhibition zone (14mm) only against Staphylococcus auricularis . As a result of combination chloroform leaves extract with each other the strongest synergistic activity exhibited during combination of Mentha longifolia with Ocimum basilicum (14 to 25mm) against Escherichia coli , while during combination of leaves extract with antibiotics the greatest synergistic activity resulted from combination of Mentha piperita with azithromycin and ampicillin separately (14 to 30mm) and (10 to 23mm) against Escherichia coli and Streptococcus mitis respectively. Escherichia coli revealed susceptibility to all leaves extract and antibiotics alone and pronounced increase in susceptibility to combination of leave extract with each other and with antibiotics. Bioautography assay showed presence number active constituents with antibacterial activity in leaves extract in which three of them identified as caffeic acid, luteolin and rosmarinic acid. Conclusion: From the result concluded that Mentha piperita showed strongest activity followed by Mentha longifolia then Ocimum basilicum alone and there activity increased in combination form.Downloads
References
Levy SB. The antibiotic paradox: How the Misuse of antibiotics destroys their curative powers. Cambridge (MA): Perseus Publishing; 2002.
Prescott H, Klein JO. Microbiology. 6th ed. USA: Macgraw Hill Publishers; 2002: 808-23.
Juliani HR, Simon JE. Antioxidant Activity of basil. In: Janick J, Whipkey A, editors. Trends in New Crops and New Uses. Alexandria, VA, USA: ASHS Press; 2002. p. 575-9.
Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003; 10:813-29.
Moghadam SM, Maleki S, Darabpour E, Motamedi H, Nejad MS. Antibacterial activity of eight Iranian plants extracts against methicillin and cefixime resistant Staphylococcus aureus strains. Asian Pac J Trop Med 2010: 262-5.
Saiful AJ, Mastura M, Zarizal S, Mazurah MI, Shuhaimi M, Ali AM. Detection of methicillin-resistant Staphylococcus aureus using mecA/nuc genes and antibiotic susceptibility profile of Malaysian clinical isolates. World J Microbiol Biotechnol 2006; 22: 1289-94.
Adwan MG, Abu-Shanab BA, Adwan KM. In vitro activity of certain drugs in combination with plant extracts against Staphylococcus aureus infections. Pak J Med 2008; 24: 541-4.
Nascimento GGF, Locatelli J, Freitas CP, Silva LG. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 2000; 31: 247-56.
Matias EFF, Santos KKA, Almeida TS, Costa JGH, Coutinho HDM. Phytochemical screening and modulation of antibiotic activity by Ocimum gratissimum L. Biomedicine Prev Nutr 2011; 1: 57- 60.
Abubakar ME. Antibacterial potential of crude leaf extracts of Eucalyptus camaldulensis against some pathogenic bacteria. Afr J Plant Sci 2010; 4(6): 202-9.
Singh B, Sahu MP, Sharma KM. Anti-inflammatory and antimicrobial activities of triterpenoids from Strobilanthes callosus Nees. Phytomed 2002; 9: 355-9.
Rabe T, Mullholland D, Staden van J. Isolation and identification of antibacterial compounds from Vernonia colorata leaves. J Ethnopharmacol 2002; 80: 91-4.
Aiyegoro OA, Okoh AI. Use of bioactive plant products in combination with standard antibiotics: implications in antimicrobial chemotherapy. J Med Plant Res 2009; 3:1147-52.
Sujana P, Sridhar MT, Josthna P, Naidu VC. Antibacterial activity and phytochemical analysis of Mentha piperita L. (Peppermint)
Ghderi P, Ahmadi R, Balkanyian F, Moridikyia A, Mahdavi E, Tavakoli P. In-vitro antibacterial activity of Bunium persicum and Mentha longifolia against Bacillus subtilis and Staphylococcus aureus. Agric Med Sci 2014: 34-6.
Kaya I, Yigit N, Benli M. Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr J Tradit Complement Altern Med 2008; 5(4): 363-9.
Ahmed AS. Antibacterial activity of Mentha piperita and Allium sativum against some of gram-ve bacteria. Al- Mustansiriyah J Sci 2012; 23(5): 39-48.
Aburjai T, Darwish R, Al-Khalil S, Mahafzah A, Al-Abbadi A. Screening of antibiotic resistant inhibitors from local plant materials against two different strains of Pseudomonas aeruginosa. J Ethnopharmacol 2001; 76: 39-44.
Nascimento FGG, Locatelli J, Freitas CP, Silva LG. antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol 2000; 31: 247-56.
Abd El-Kalek HH, Mohamed AE. Synergistic effect of certain medicinal plants and amoxicillin against some clinical isolates of methicillin
Sibanda T, Okoh AI. The challenges of overcoming antibiotic resistance: Plant extracts as potential sources of antimicrobial and resistance modifying agents. Afr J Biotechnol 2007; 6:2886-96.
Gibbons S. Phytochemicals for bacterial resistance: Strengths, weaknesses and opportunities. Planta Med 2008; 74: 594-602.
Akroum S, Bendjeddou D, Satta D, Lalaoui K. Antibacterial activity and acute toxicity effect of flavonoids extracted from Mentha longifolia. Am.-Eurasian J Sci Res 2009; 4(2): 93-6.
Zg
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
How to Cite
