Hepatitis C Virus (HCV) RNA Quantification and Typing for the Identification of HCV Genotypes in clinical isolates

Authors

  • Narotam Sharma Research Scientist, Molecular Research Laboratory, Department of Biochemistry, SGRRIM&HS, Patel Nagar Dehradun (U.K)
  • Meenakshi Bahuguna Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001
  • Sneha Rawat Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001
  • Neelam Panwar Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001
  • Priyanka Chauhan Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001
  • Rakhi Kaushik Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001
  • Satish Chandra Nautiyal Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001
  • R K Singh Central Molecular Research Laboratory, Biochemistry Department, SGRRIM&HS, Patel Nagar, Dehradun, Uttarakhand-248001

DOI:

https://doi.org/10.7439/ijbar.v6i6.2108

Abstract

The frequency of infection with the major genotypes of hepatitis C virus (HCV) was investigated in the current study. 30 serum specimens were quantified for HCV RNA viral load and their titer values were observed with the usage of Real Time PCR in-between 1.05x101-9.58x107IU/ml. HCV genotype 3a was found in 17(56.6%) cases with highest prevalence. Genotypes 1a, 1b, 2a, 2b ,3b ,4, and 5a was found 2(6.6%),15(50%),5(16.6%),15(50%),2(6.6%),2(6.6%),2(6.6%) respectively. The findings also included that HCV viral RNA titre was elevated in HCV genotype 3a, assuming that HCV genotype 3a is the most replicated virus and need to be monitored thoroughly during diagnosis. Although type 1a, 1b, 2a, 2b, 3b, 4,5a were also present but in lower proportion.

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Published

2015-06-30

Issue

Vol. 6 No. 6 (2015): Jun

Section

Original Research Articles

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How to Cite

Hepatitis C Virus (HCV) RNA Quantification and Typing for the Identification of HCV Genotypes in clinical isolates. (2015). International Journal of Biomedical and Advance Research, 6(6), 466-469. https://doi.org/10.7439/ijbar.v6i6.2108
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