Validation of the Friedewald Formula in Type II diabetes mellitus : An Indian perspective study
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https://doi.org/10.7439/ijbar.v6i2.1581Abstract
Introduction : Diabetes mellitus is characterized by hypertriglyceridemia and abnormal lipoprotein composition. Since LDL is a modifiable risk factor for cardiovascular complications in diabetes, its measurement is recommended in routine clinical practice. Objective of the study is to assess the accuracy of serum LDL concentration estimation by Friedewald formula and by homogenous method in normal and diabetic patients. Materials and Methods : Fasting blood samples were collected from 300 subjects (109 normal persons 101 prediabetics, 90 diabetics). Glucose, total cholesterol, LDL, HDL were estimated spectrophotometrically and LDL was calculated by Friedewald formula. LDL calculated by Friedewald formula was compared with that obtained by direct assay. Results : LDL determined by direct assay correlated highly with calculated LDL in all subjects irrespective of fasting glucose value. Estimated LDL was higher than calculated LDL in normal and diabetic group, indicating the fact that calculated LDL underestimates the true LDL levels irrespective of fasting glucose levels. Further, the difference in the means were significantly high in all the 3 groups with significance being highest in the diabetic group (p 0.001) Conclusion : it can be concluded that estimation of LDL is always a better investigation technique than following calculations for LDL estimation, considering the importance of patient care in treatment of lipid disorders in diabetes mellitus. Key words : Friedewald formula, Direct LDL estimation, diabetes mellitusDownloads
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