IN VITRO-STUDY OF EFFECT OF HERBAL SYRUP OF MEDICINAL PLANT EXTRACT AGAINST ESBL PRODUCING KLEBSIELLA PNEUMONIA CAUSING UTI INFECTION

Abstract

This study was carried out from 8^th Jan 2010 to 8^th Feb 2011. A total of 60 consecutive Klebsiella recovered during the study period in 100 urine sample of UTI patients. 22 isolates were ESBL producer and 38 isolates were non-ESBL producers. The prevalence of extended spectrum β-lactamase producing Klebsiella in urine sample of UTI patients was 22%. Detection of extended spectrum β-lactamase producing Klebsiella in urine sample of UTI patients was carried out by double disc diffusion method on Muller Hinton Agar. A susceptibility disk containing Piperacillin\ Tazobactum was placed as the inhibitor of β-lactamase in the center of the plate, Piperacillin were placed 30 mm from the Piperacillin\Tazobactum disk. Enhancement of zone of inhibition of disc of Piperacillin alone towards the disc containing Piperacillin\ Tazobactum, showing a figure of eight impression were considered as ESBL producer. All recovered isolates were resistant against ampicillin, amoxicillin, ceftazidime, ceftriaxone, tetracycline, chloramphenicol, gentamycin, and cefotaxime and sensitive against impenem, amikacin, and ciprofloxacin and meropenem. Antimicrobial activity of medicinal plants Euphorbia heterophylla and Acalypha indica were assessed for ESBL producing Klebsiella pneumoniae.