IMPACT OF EXTRACELLULAR CALCIUM ALONG WITH ASCORBIC ACID ON CELLULAR REACTIVATION AND INSULIN SECRETION IN DIABETIC MIN6 CELLS

Authors

  • Priyanka Sharma School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalaya, Bhopal, Madhya Pradesh
  • Archana Tiwari School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalaya, Bhopal, Madhya Pradesh

DOI:

https://doi.org/10.7439/ijbar.v2i12.236

Abstract

Diabetes is not a single disease but characterized by a group of syndromes. Oxidative stress and defects in insulin secretory pathway are the major problems associated with type 2 diabetes. Extracellular Ca2+ influx is the major trigger which stimulates insulin secretion in pancreatic β cells. In this study we combined the most common antioxidant ascorbic acid with extracellular calcium to observe their cooperative effect on diabetic MIN6 cells. Our results demonstrated firstly the major role of ascorbic acid individually as well as with calcium in cell viability as compared to individual calcium supplementation. Secondly, the combined concentration of ascorbic acid and calcium leads to maximum increase in insulin level in dose dependent manner upto 5mM ascorbic acid and decrease beyond this concentration.

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Published

2011-01-01

Issue

Vol. 2 No. 12 (2011): Dec

Section

Original Research Articles

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How to Cite

IMPACT OF EXTRACELLULAR CALCIUM ALONG WITH ASCORBIC ACID ON CELLULAR REACTIVATION AND INSULIN SECRETION IN DIABETIC MIN6 CELLS. (2011). International Journal of Biomedical and Advance Research, 2(12), 489-493. https://doi.org/10.7439/ijbar.v2i12.236
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