RP-HPLC Method for Simultaneous Estimation of Levofloxacin Hemihydrate and Ornidazole from Tablet Formulation
DOI:
https://doi.org/10.7439/svmgeb74Keywords:
Levofloxacin hemihydrate, Method validation, Ornidazole, Reversed phase HPLCAbstract
An accurate, simple, reproducible and precise reverse phase high-performance liquid chromatographic method for the determination of levofloxacin hemihydrate and ornidazole has been developed and validated. The separation of levofloxacin, ornidazole and fluconazole (internal standard) was achieved on a Luna C18 (5μ, 250 mm X 4.60 mm i.d.) column using UV detection at 254 nm. The mobile phase was consisted of 0.5 % triethylamine in water and acetonitrile (65: 35) pH adjusted to 3.0 with 5 % phosphoric acid. The flow rate was 0.8 ml/min. The retention time of levofloxacin hemihydrate and ornidazole were 3.17 min and 6.63 min respectively. The linearity of levofloxacin and ornidazole were found to be 4 – 24 mcg/ml (r2= 0.9991) and 8 – 48 mcg/ml (r2= 0.9992), respectively. The method has been successively applied to pharmaceutical formulation. No chromatographic interferences from the tablet excipients were found. The method has been shown to be linear reproducible, specific and rugged.
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