Development of First Order Derivative UV-Spectrophotometric Method for Determination of Tamsulosin Hydrochloride in Bulk and in Formulation

Abstract

A simple, rapid, accurate and economical “First order UV-derivative spectrophotometric” method has been developed for estimation of Tamsulosin (TMS) from bulk and pharmaceutical formulation. The λ max of TMS in methanol and water was found to be 280 nm. The same spectrum was derivatised in to first order derivative; showed maximum amplitude of the trough at 299 nm. The drug follows linearity in the concentration range 10 - 90 µg/ml with correlation coefficient value 0.997. The value of correlation coefficient (r²) greater than 0.99 indicate good linearity response in the above-mentioned range. The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 99.58 % was found in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels i.e., 80%, 100% and 120 %. The % recovery was found to be in the range 99.00%– 101.63%. The low values of % R.S.D. are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The % R.S.D. value less than 2 indicate that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts. The results did not show any statistical difference between operators suggesting that method developed was rugged.