A novel validated RP-HPLC method for the estimation of canagliflozin in bulk and pharmaceutical dosage forms

Authors

  • Vijaya Lakshmi Marella Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University
  • Aisha Syed Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University
  • Lakshmi Prasanna M Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University
  • Buchi Naidu Nalluri Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University

DOI:

https://doi.org/10.7439/ijapa.v7i3.4350

Keywords:

Brenner tumour, immunohistochemistry, malignant Brenner

Abstract

The objective of the present study was to develop a simple, specific and accurate reverse phase high performance liquid chromatographic method for the determination of Canagliflozin in bulk and pharmaceutical dosage forms. The method is optimized on an inertsil ODS-3(250.6mm, 5) column with a mobile phase combination of 0.02% Formic acid: Acetonitrile (40:60) at a flow rate 1.2ml/min and the eluents were monitored at 230nm. Under these LC conditions Canagliflozin peak was eluted at 4.4 min. The developed method was validated as per ICH guidelines. The calibration curve was linear over a concentration range of 10-50g/ml (R 2 =0.999) and the mean percentage assay was found to be 98.2. The statistical data proved that proposed method is accurate, precise and reproducible. The method which is LC-MS compatible can be adopted in the routine analysis of Canagliflozin in bulk and pharmaceutical dosage forms.

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Author Biographies

  • Vijaya Lakshmi Marella, Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University
    Department of pharmaceutical analysis
  • Aisha Syed, Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University
    Department of pharmaceutical analysis
  • Lakshmi Prasanna M, Department of Pharmaceutical Analysis, KVSR Siddhartha College of Pharmaceutical Sciences, Krishna University
    department of pharmaceutical analysis

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Published

2017-09-30

Issue

Vol. 7 No. 3 (2017): Jul-Sep

Section

Research Articles

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How to Cite

1.
A novel validated RP-HPLC method for the estimation of canagliflozin in bulk and pharmaceutical dosage forms. Int J of Adv in Phar Ana [Internet]. 2017 Sep. 30 [cited 2024 Oct. 18];7(3):24-7. Available from: https://ssjournals.co.in/index.php/ijapa/article/view/4350

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