Comparitive study of UV-Visible spectrophotometry and high performance liquid Chromatography methods for quantitative estimation of paracetamol in a tablet formulation

Authors

  • Chelamalla Radhika CMR COLLEGE OF PHARMACY Kandlakoya (V), Medchal Road Hyderabad - 501 401. Andhra Pradesh. INDIA.
  • Akena Venkatesham University College of Pharmaceutical Sciences, Kakatiya University, Warangal-506009,A.P,

DOI:

https://doi.org/10.7439/ijapa.v5i2.2221

Keywords:

Succinylated gelatin, Hydroxyethyl starch, Cardiopulmonary bypass, Coronary artery bypass, PRBC, CVP

Abstract

The objective of present study was to analyse the comparision between high performance liquid chromatography(HPLC) and UV-Spectrophotometry for quantitative determination of paracetamol in a marketed formulation. Rp-HPLC method involved a reversed-phase XBD C18 column thermostated at 25C, UV detection at 230nm, flow rate of 1.0ml/min and a mobile phase acetonitrile-water(25:75) was used. These methods showed good linearity over the concentration range of 220 ?g/mL. The linearity was obtained for paracetamol by reverse phase high performance liquid chromatography (RP-HPLC) R 2 =0.993 and by UV - Spectrophotometry R 2 =0.977. The precision and recoveries of paracetamol for HPLC and UV-Spectrophotometry methods were in the range of 99.00 -100.10 % and 99.71-100.95 %. Among these two methods high performance liquid chromatography method of analysis showed reliable results for quantitative etermination of paracetamol in a tablet formulation.

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Published

2015-06-29

Issue

Vol. 5 No. 2 (2015): Apr-Jun

Section

Research Articles

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How to Cite

1.
Comparitive study of UV-Visible spectrophotometry and high performance liquid Chromatography methods for quantitative estimation of paracetamol in a tablet formulation. Int J of Adv in Phar Ana [Internet]. 2015 Jun. 29 [cited 2026 Jan. 3];5(2):47-50. Available from: https://ssjournals.co.in/index.php/ijapa/article/view/2221
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