Bioanalytical method validation of fenofibrate by hplc using human plasma

Abstract

The bioanalytical method validation of fenofibrate was performed with all the basic parameter by HPLC using human plasma. This research paper describes a specific procedure for validation of Fenofibrate in human plasma. The mobile phase used was simple and column friendly and was sufficiently sensitive to quantify Fenofibrate in amounts as low as 0.095g/ml. This limit of quantitation (LOQ) was determined as the lowest concentration with a coefficient of variation lower than 20% and the total accuracy of the method ranged from 101.99 to 107.41%. The LOQ of this method is better than those of other reported methods. The calibration curve plotted concentration versus area and was linear from 0.095 g/ml to 19.924 g/ml, having r ² greater than 0.98 during the course of validation. The above method is valid for the analysis of drug in human plasma. The method with slight modification could be used for the drug analysis in various dosage forms.