Determination of Rivaroxaban in pure, pharmaceutical formulations and human plasma samples by RP-HPLC
DOI:
https://doi.org/10.7439/ijapa.v5i3.2800Abstract
A simple, sensitive and rugged reverse-phase high performance liquid chromatographic method has been developed and validated for the determination of rivaroxaban in pure, pharmaceutical formulations and in spiked human plasma sample. The separation of rivaroxaban and internal standard was achieved on XDB C18 (150 x 4.6) mm column. Mobile phase employed for the study is the mixture of water and acetonitrile in gradient programme. A flow rate of 1 ml/min was found optimum for the study. Linearity of the proposed method was found to be in the range of 0.05 g/ml to 20 g/ml with r 2 =0.9999. The limit of detection and limit of quantification of the proposed method are found to be 0.015 g/ml and 0.046 g/ml, respectively. Intra-day and inter-day assay relative standard deviations were determined and found to be less than 2.0 %. The method has been applied successfully for the determination of rivaroxaban in its pharmaceutical formulations and in spiked human plasma samples.Downloads
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