Development and validation of Novel UV-Spectrophotometric method for estimation of Cyamemazine Tartarate for simple, novel, rapid and cost effective analysis

Abstract

Simple, novel, rapid and cost effective UV-spectrophotometric method has been developed and validated for estimation of CYMT in bulk and tablet formulation. CYMT is a Phenothiazine derivative from the class of typical antipsychotic and a new drug for the treatment of Schizophrenia. As no analytical method was seen in literature for determination of CYMT; we report here, development and validation of simple, novel UV-spectrophotometric method for analysis of CYMT. Drug was found to be soluble in water and was stable for more than 72 h in same solvent when tested for bench top stability; being ideal solvent, spectroscopic studies were carried out using distilled water and detection as well as quantitation was performed at wavelength maximum of 267 267.21 267.21 nm. Linearity curve for developed method was generated by measuring absorbance at specified wavelength maximum and plotting it against concentration. The method followed linearity in range of 2 - 12 ?g/mL with a correlation coefficient of 0.999. The mean recovery of 98.97 reflects accuracy for developed method and the method precision was found to be well within acceptable limits. The developed method was tested and validated for various parameters as per USP requirements and recent ICH guidelines (addendum 2005). The present UV-spectrophotometric estimation for CYMT was proved to be statistically accurate, precise, and sensitive. The applicability of method can be extended towards rapid routine determination of drug in bulk and pharmaceutical formulations and it has the unique importance of being first simple analytical and UV-Spectroscopic method for the determination of CYMT in bulk and in pharmaceutical formulation.