New validated RP-HPLC method for simultaneous estimation of chlorpromazine and trihexyphenidyl HCl in tablets
DOI:
https://doi.org/10.7439/ijapa.v4i4.103Keywords:
Hemoglobinopathies, Sickle cell Anemia - Thalassemia Disease, African populationAbstract
A simple, specific and precise reverse phase high performance liquid chromatographic method was developed and validated for simultaneous estimation of Chlorpromazine and Trihexyphenidyl HCl in tablets. Quantification was achieved by using a reverse-phase C18 column (Inertsil ODS 3V, 250 mm x 4.6 mm; 5) at 31 o C. The mobile phase consisted of a mixture of ammonium acetate buffer and methanol in the ratio of 15:85 v/v at a flow rate of 1.2 mL/min. The retention times of Chlorpromazine and Trihexyphenidyl HCl were found to be 3.237 min and 5.260 min respectively. The developed method was validated as per ICH Guidelines for linearity, accuracy, precision, detection limit, quantification limit, ruggedness, robustness, specificity and system suitability. The percentage recoveries for both of the drugs from their tablets were found to be 100.34 % and 99.80 % respectively. The method may successfully be employed for the simultaneous determination of Chlorpromazine and Trihexyphenidyl HCl in pharmaceutical tablet dosage forms.Downloads
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