Development of stability indicating assay method for the simultaneous estimation of Metformin hydrochloride and Glipizide by RP-HPLC method

Authors

  • Manoj S. Charde Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • G. Yadav Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • A. S. Welankiwar Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • Jitendra K. Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • R. P. Marathe Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra

DOI:

https://doi.org/10.7439/ijapa.v4i3.95

Keywords:

Blood Lactate, Anaerobic Power, Fatigue index

Abstract

A simple, sensitive an isocratic RP-HPLC method for the estimation of Metformin Hydrochloride (MET) and Glipizide (GPZ) in combined dosage form using Inertsil C8 column (250.6 mm, 5 ) in an isocratic mode with mobile phase comprising Acetonitrile: Water (70:30) and one drop of Triethylamine. The flow rate was 1.0 ml/min and effluent was monitored at 222 nm. The retention times were found to be 3.40 min for MET and 4.44 min for GPZ. The assay exhibited a linear dynamic range of 100-500 g/ml of MET and 1-5 g/mL for GPZ .The calibration curves were linear (r = 0.9985 for MET and r = 0.9955 for GPZ) over the entire linear range. Recovery was found to be 99.94 % + .17 for MET and 99.61 % + 0.89 for GPZ. % RSD of system precision were observed 0.0429 for MET and 0.7212 for GPZ.

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Author Biography

  • Manoj S. Charde, Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
    Assitant Professor

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Published

2014-10-01

Issue

Vol. 4 No. 3 (2014): Jul-Sep

Section

Research Articles

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How to Cite

1.
Development of stability indicating assay method for the simultaneous estimation of Metformin hydrochloride and Glipizide by RP-HPLC method. Int J of Adv in Phar Ana [Internet]. 2014 Oct. 1 [cited 2025 Mar. 15];4(3):113-9. Available from: https://ssjournals.co.in/index.php/ijapa/article/view/1396
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