Secondary metabolites screening from in-vitro cultured Rauwolfia tetraphylla by HPTLC-MS: A special emphasises on their antimicrobial applications

Abstract

The current study designed at evaluating the phytochemical, trace metal concentration and antimicrobial properties were screened by the ethanolic extracts of in-vitro cultured medicinal plant Rauwolfia tetraphylla . The In-vitro shoots proliferation from nodal explants of R. tetraphylla using Murashige and Skoog (MS) medium containing 0.1mM of NAA and 0.25mM of BAP was effectively induce the shoot buds. The phytochemical analysis of cultured plant extracts revealed the presence of steroids, reducing sugars, sugars, alkaloids, phenols, flavonoid, saponins, tannins and amino acids. In continuously, we assessed by HPTLC coupled with mass spectrum, based on the mass spectrum were easily identified the major compounds such as 3-isoreserpine, ajmalicine, ajmaline, reserpine and yohimbine from R. tetraphylla . Metal contents of plant samples, Cd, Cr, Cu, Fe, Ni, Pb and Zn concentrations are BDL, BDL, 0.12, 0.68, BDL, BDL and 0.62 mg kg-1, respectively. The ethanol extraction of in-vitro R. tetraphylla inhibits the growth of bacteria and fungi to a greater extent.