Assessment of phytochemical constituents, trace metals and antimicrobial efficacy of holy plant Couroupita guianensis, Southern India

Abstract

Photochemical constituents, trace metals concentration and antimicrobial activity of (three different extract such as petroleum ether, chloroform and ethanol) C. guianensis leaves were investigated. The phytochemical screening of the crude extract revealed the presence of steroids, triterpenes, alkaloids, phenols, flavonoids, saponins and tannins. The leave powder was subjected to analyse the trace metals using 797 VA Computrace voltametry, Metrohm. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn were BDL, 0.02, 0.32, 0.89, BDL, BDL and 0.54 mg kg ^-1 , respectively. The three different solvents extract of C.guianensis were analyzed for in-vitro antimicrobial activity against certain pathogens and the zone of inhibition were compared with positive control such as Methicillin 10 mcg and Itraconazole 10 mcg.The Enterococcus fecalis and Trichophyton rubrum were most sensitive (19 mm) while smallest inhibition were recorded in Micrococcus luteus and Cryptococcus sp. (7 mm).