Development and validation of an RP-HPLC method for determination of Cefixime and Dicloxacillin in tablet dosage form

Authors

  • Prabhudev M S Department of Pharmaceutical Chemistry, RMES's College of Pharmacy Gulbarga (Karnataka)
  • H J Kallur Department of Pharmaceutical Chemistry, RMES's College of Pharmacy Gulbarga (Karnataka)
  • Kishoresingh C Department of Pharmaceutical Chemistry, RMES's College of Pharmacy Gulbarga (Karnataka)
  • Siddanna A Durgad Department of Pharmaceutical Chemistry, RMES's College of Pharmacy Gulbarga (Karnataka)
  • Hariprasanna R. C. Department of Pharmaceutical Chemistry, RMES's College of Pharmacy Gulbarga (Karnataka)
  • Pramod Subhash Sawanth Department of Pharmaceutical Chemistry, RMES's College of Pharmacy Gulbarga (Karnataka)

DOI:

https://doi.org/10.7439/ijapa.v3i2.45

Abstract

A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Cefixime and Dicloxacillin in bulk drug and tablet dosage form. Sepration was achieved by capcell pack C-18 column having 250 mm4.6 mm i.d. in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen phosphate: methanol (25:75), adjusted to pH 4.5 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluents were monitored at 232 nm. The retention time of Cefixime and Dicloxacillin were 3.19 min and 6.68 min respectively. The linearity for Cefixime and Dicloxacillin were in the range of 10-100 g/ml. The recoveries of Cefixime and Dicloxacillin were found in the range of 99.63-99.95 % and 99.58-99.98 % respectively. The proposed method was validated as per ICH and USP guidelines and successfully applied to the estimation of Cefixime and Dicloxacillin in bulk drug and tablet dosage form.

Downloads

Download data is not yet available.

Downloads

Published

2013-07-14

Issue

Vol. 3 No. 2 (2013): Apr-Jun

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.

b)Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

After acceptance of the article, the author has to submit copyright form signed by all authors.

How to Cite

1.
Development and validation of an RP-HPLC method for determination of Cefixime and Dicloxacillin in tablet dosage form. Int J of Adv in Phar Ana [Internet]. 2013 Jul. 14 [cited 2026 Jan. 3];3(2):42-50. Available from: https://ssjournals.co.in/index.php/ijapa/article/view/1369
Crossref
Scopus
Google Scholar
Europe PMC