Estimation of Pazopanib Hydrochloride in Tablet Dosage Forms By RP-HPLC

Abstract

A simple, precise, rapid and accurate RP- HPLC method was developed for the Estimation of Pazopanib HCl (PZP) in tablet dosage forms. An XTerra RP C ₁₈ , (250X4.6 with 5 microns particle size) and the mobile phase, consisting of 0.03M KH ₂ PO ₄ in water adjusting the pH-3.2 with O-Phosphoric Acid: Acetonitrile in ratio of 70:30 v/v and water: Acetonitrile (50:50 v/v) was used as diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 267 nm. The retention time was 7.392 for Pazopanib HCl. The detector response was linear in the concentration of 20-240 g/mL for PZP. The respective linear regression equation being Y (528142.1276 = 172694.049x + 428066.7611 for PZP. The Limit of Detection (LOD) is 0.10 and The Limit of Quantification (LOQ) is 0.30 for PZP respectively. The % assay of PZP was found out to be 99.49%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of PZP in bulk drug and in its pharmaceutical dosage forms.