Development of UV spectrophotometric methods and validation for estimation of furosemide in bulk and tablet dosage form by absorbance maxima and Area Under the Curve method
DOI:
https://doi.org/10.7439/ijap.v5i6.3791Keywords:
Corchorus tridens L, soxhlet apparatus, antimicrobial activity, UV, FT-IR, GC-MS analysis.Abstract
The present work was to develop two simple UV spectrophotometric methods for simultaneous estimation of furosemide (FUR) in bulk and tablet dosage form and validate as per ICH guidelines. Method A is absorption maxima method in which ?max was found to be 277 nm. Method B is area under the curve (AUC) in which area in the wavelength range of 258.40 nm 293.80 nm was selected for analysis of furosemide .Linearity was observed in the concentration range 5-25?g/ml (r2 =0.999) for both the methods. The % assay for the marketed formulation for absorption maxima and area under the curve method was found to be 99.16%, and 99.20% respectively. The methods were validated with respect to linearity, precision and accuracy studies. Recovery studies for absorption maxima, and area under the curve was found to be 100.46%, and 100.86% respectively. The developed methods were validated for linearity, precision, accuracy, LOD and LOQ as per ICH guidelines. Both the methods were found to be linear within the conc. Range of 5-25?g/ml for furosemide. The present methods were found to be simple, linear, precise, accurate and sensitive and can be used for routine quality control analysis for the estimation of furosemide in bulk and tablet dosage form.Downloads
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Published
2016-12-30
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1.
Development of UV spectrophotometric methods and validation for estimation of furosemide in bulk and tablet dosage form by absorbance maxima and Area Under the Curve method. Int J Adv Pharm [Internet]. 2016 Dec. 30 [cited 2024 Oct. 18];5(6):160-7. Available from: https://ssjournals.co.in/index.php/ijap/article/view/3791