HPTLC fingerprint profile of methanol extract of the marine red alga Portieria hornemannii (Lyngbye) (Silva)
DOI:
https://doi.org/10.7439/ijap.v5i3.3209Abstract
The present study was aimed to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of methanol extracts of marine red alga Portieria hornemannii (Lyngbye) (Silva). A HPTLC method for the separation of the active constituents in extracts has been developed and TLC of these extracts on silica gel pre-coated aluminum plates of Merck by the automatic TLC applicator and using the solvent system n-hexane:ethyl acetate (60:40 v/v) was performed. HPTLC profiling of the extract confirm about the presence of various phytochemicals. HPTLC finger print scanned at 490 nm for methanol and ethyl acetate algal extracts revealed 13 peaks with Rf values in the range of 0.16 to 0.99 respectively. The HPTLC method for routine quality control of present species can be carried out using this method for extracts of plant and serve in qualitative, quantitative and was appropriate for standardization of the extract.Downloads
References
Wylie C R and Paul V J. Chemical defenses in the three species of Sinularia
(Coelenterats Alcyonacea) affects against generalist predator and butterfly fish Chetodon.
Unima culatus Block. Journal Exp Mar Biol Ecol (1989).129, 141-160.
Thennarasan S, Murugesan S, Chidambaranathan N and Sivamurugan. V. Analgesic, Anti-inflammatory and Antipyretic Activity of the Methanol Extracts of Brown Alga Lobophora variegata (J.V.Lamouroux) Womersley ex E.C.Oliveir. American Journal of Phytomedicine and Clinical Therapeutics (2016). 4(2): 042-057.
Baker J T, and Murphy, V. Handbook of marine science, compound from marine organisms. Cleveland Ohio (1976). CDR Press.
Padmakumar K, and Vethanayagam R R. Screening Indian marine sponges for antibiotics activity. Journal Toxic: Toxin Rev (1995). 14, 207.
Gupta RK, Medicinal & Aromatic plants, CBS publishers & distributors, 1st edition, (2010), 116-117.
Pandey G, Dravyaguna Vignyan, Vol. 2, Chaukhamba Krishnadas Academy, Varanasi, (2004). 158-162.
Kirtikar KR, Basu BD.,Indian Medicinal Plants. Edn 2, Vol. 1, International Book Distributors, Deharadun, (2005), 649-651.
Sushma GS, Archana Devi B, Madhulatha CH, Uday Kumar K, Harathi P, Siva Subramaniam N. Preliminary phytochemical screening and HPTLC fingerprinting of leaf extracts of Ficus nervosa Heyne ex Roth, Journal of Chemical and Pharmaceutical Research, (2013). 5(3): 98-104.
Thennarasan S, Murugesan S and TS. Subha. HPTLC finger printing profile of brown alga Lobophora variegata (J.V. Lamouroux). Journal of Chemical and Pharmaceutical Research (2014). 6(1):674-677.
Pandithurai M, Murugesan S, Sivamurugan V and Lakshmisundram R. Chromatographic Fingerprint Analysis of Spatoglossum asperum J.Agardh by HPTLC Technique. American Journal of Modern Chromatography (2015). 2(1): 7-15.
Bhuvaneswari S, Murugesan S. Biochemical composition of seaweeds along southeast coast of Tamilnadu, India. International Journal of Biology and Pharmacy and Allied Sciences (2013). 2(7): 1430-1436.
Murugesan S, Bhuvaneshwari S. Evaluation of antioxidant activity of methanol extracts of red algae Chondrococcus hornemannii and Spyridia fusiformis. International Journal of Advances in Pharmaceutics (2016). 5 (1): 8-11.
Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis.3rd
Edition (1998.), Chapman and Hall Pub, London, UK.
Kpoviessia D S S, Gbaguidia F, Gbenoua J, Accrombessia G, Moudachiroua M, Rozetd E,
Hubertd P and Leclercq JQ. Validation of a method for the determination of
sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas
chromatography. J Pharm Biomed Anal (2008). 48: 1127-1135.
Halinski P, Szafranek J, Szafranek BM, Goe?biowski M, Stepnowski P. Chromatographic fractionation and analysis of the main components of eggplant (Solanum melongena L.) leaf cuticular waxes. Acta Chromatographica (2009). 21: 127-137.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
