Simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form by absorption ratio method using UV-Vis spectrophotometry

Authors

  • R. D. Chakole Government College of Pharmacy, Kathora Naka, Amravati-444604, Maharashtra
  • Manoj C. Government College of Pharmacy, Kathora Naka, Amravati-444604, Maharashtra

DOI:

https://doi.org/10.7439/ijap.v3i1.1416

Abstract

A simple, precise, reproducible, accurate, economical and rapid UV-VIS Spectrophotometric method have been developed and validated for the simultaneous estimation of ATN and CTN in tablet dosage form. This paper describes the absorption ratio method as a quantification parameter. The absorption ratio method (ARM) involves measurement of absorbance of sample solution is measured at 240.0 nm (Isobestic Point) and 251.0 nm (? max of CTN) and based on E 1% 1cm values at these wavelengths two set of equations were framed. The developed method obeys the beers law in the concentration range of 40-80g/mL for ATN and 10-50 g/mL for CTN. The recovery studies shows %RSD for ATN 0.21 and for CTN 1.34 by ARM method. The results of analysis have been validated statistically for accuracy, precision, repeatability, specificity and ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form.

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Published

2014-03-31

Issue

Vol. 3 No. 1 (2014): Jan-Feb

Section

Research Articles

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How to Cite

1.
Simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form by absorption ratio method using UV-Vis spectrophotometry. Int J Adv Pharm [Internet]. 2014 Mar. 31 [cited 2024 Sep. 8];3(1):19-27. Available from: https://ssjournals.co.in/index.php/ijap/article/view/1416